The biofilm eradication activity of c-10 massoialactone against staphylococcus aureus

C-10 massoaialactone is a major component of stem bark essential oils Mas-soia aromatica Becc, which has potential as antibacterial and antifungal but antibiofilm activity against S. aureus has never been reported. The discovery of anti-biofilm drug candidates is needed to overcome infections associated with biofilms. This study aims to determine the eradication activity of C-10 massoialactone against S. aureus biofilms. Antibacterial testing and eradication activity of S. aureus biofilms was determined using the microtiter broth method. The effectiveness of C-10 massoilactone against S. aureus biofilm was analyzed by calculating the value of minimum biofilm eradication con-centration. The mechanism of action of C-10 massoilactone against S. aureus biofilms was tested using scanning electron microscopy (SEM). C-10 mas-soilactone 1 gives antibacterial activity of 86.80 ± 0.01 and can eradication S. aureus biofilm by 68.98 ± 0.01 and not much different from the eradication activity of chloramphenicol drug control. The results also pro-vide evidence that C-10 masoaialactone can damage the extracellular poly-meric substance (EPS) matrix and S. aureus biofilm morphology. Therefore, C-10 masoaialactone is very potential to be developed as a candidate for a new antibiofilm drug against S. aureus biofilm.