Isolation of secondary metabolites from paku atai merah (Angiopteris ferox copel) Tubers

Aim and Background: Paku atai merah (Angiopteris ferox Copel) tuber is a type of fern plant that has been used empirically as a traditional medicine in society. The part of the plant that is often used is the tuber. But scientifically this plant is not known for its secondary metabolite content. The purpose of this study was to determine the secondary metabolites found in paku atai merah tubers. Methods: The sample was extracted by maceration method with ethanol solvent, fractionated by conventional column chromatography and characterized by Nuclear Magnetic Resonance (NMR). Results: The fractionation using the chromatographic column was obtained as many as 13 fractions. From the fraction, the optimal separation from fraction 4 (F4) was obtained. The results of the separation again from F4 obtained a total of 17 sub-fractions (F1-F17). Sub-fraction of F7 and F8 showed good purity and the results of characterization showed that F7 and F8 were the same compounds, namely Angiopteroside compounds. Conclusion: This study provides a contribution that the Angiopteris ferox Copel species contains a secondary metabolite in the form of Angiopteroside which can be developed in the discovery of new drugs. © 2021 Elsevier B.V., All rights reserved.