Bax mRNA Expression as A Potential Biomarker of Placental Apoptosis in Early-onset Preeclampsia

Iqbal, Muhammad Javedh and Hadiati, Diah Rumekti and Heriyanto, Didik Setyo (2023) Bax mRNA Expression as A Potential Biomarker of Placental Apoptosis in Early-onset Preeclampsia. Indonesian Biomedical Journal, 15 (3). pp. 262-268. ISSN 20853297

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Abstract

BACKGROUND: Early-onset preeclampsia is characterized by higher oxidative stress and apoptosis level than late-onset one. Studies comparing the expression of the Bcl-2 family protein in early and late-onset preeclampsia are still lacking and show inconclusive evidence. This study aimed to compare the expression of Bax and Bcl-2 messenger RNA (mRNA) as a biomarker of placental apoptosis between early-onset and late-onset preeclampsia. METHODS: A cross-sectional study was conducted using formalin-fixed, paraffin-embedded preeclamptic placental samples and dividing them into early-onset and late-onset preeclampsia groups. Bax and Bcl-2 mRNA expressions were assessed using the quantitative real-time polymerase chain reaction method. Apoptosis was assessed through DNA fragmentation examination by the ligation-mediated real-time polymerase chain reaction method. RESULTS: Thirty early-onset and 30 late-onset preeclamptic placental samples were included. The mean fold change Bax mRNA in early-onset was higher than in late-onset preeclampsia (6.02±3.59 vs. 2.82±1.97; p=0.00). The mean fold change Bcl-2 mRNA early-onset was not different from late-onset preeclampsia (31.20±17.94 vs. 31.01±27.60; p=0.98). The mean DNA fragmentation cycle threshold in early-onset preeclampsia was lower than in late-onset preeclampsia (28.07±0.64 vs. 30.63±0.96; p=0.00). A weak negative correlation exists between fold change Bax mRNA and DNA fragmentation cycle threshold (r=-0.30; p=0.02). CONCLUSION: Bax mRNA showed significant correlation in DNA fragmentation compared to Bcl-2 mRNA; hence, might show more role in apoptotic pathway. Early-onset preeclampsia has higher Bax mRNA relative expression and apoptosis than late-onset preeclampsia. Therefore, Bax mRNA can be potential biomarker in early-onset preeclampsia.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: mRNA, Bax, Bcl-2, apoptosis, DNA fragmentation, early-onset, preeclampsia
Subjects: R Medicine > RB Pathology
R Medicine > RG Gynecology and obstetrics
Divisions: Faculty of Medicine, Public Health and Nursing > Public Health and Nutrition
Depositing User: Annisa Fitria Nur Azizah Annisa Fitria Nur Azizah
Date Deposited: 04 Jul 2024 04:08
Last Modified: 04 Jul 2024 04:08
URI: https://ir.lib.ugm.ac.id/id/eprint/2690

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