Optimization of a New Ultrasound-Assisted Extraction Method of Caffeic Acid from the Aerial Parts of Coriandrum sativum by Using Experimental Design and Ultra-Performance Liquid Chromatography

Coriander (Coriandrum sativum L.) is among the most widely used medicinal and aromatic plants. It is well known for its multiple health benefits, most of which are correlated with its phenolic composition. Four phenolic compounds were identified in the extracts of aerial parts of coriander extracts, including caffeic acid, isoquercitrin, quercetin-3-O-glucuronide, and rutin. Caffeic acid was the major compound in the extracts. A Box–Behnken Design (BBD) was employed in conjunction with the response surface methodology (RSM) to develop an ultrasound-assisted extraction method for the determination of phenolic compounds in the aerial parts of coriander using the level of caffeic acid as the target response. The following working variables were evaluated: methanol level in the extraction solvent, temperature, sonication time, and liquid-to-solvent ratio. It was found that the methanol concentration is the most significant factor that influences the recovery of caffeic acid. The optimal extraction conditions were: 10 min as the extraction time, 70 °C as the temperature, 50 for methanol in water as the solvent, and 6.51 mL of solvent per gram of sample. The repeatability and reproducibility were calculated and RSD values below 6 were obtained in both cases. The new method was employed for the extraction of real coriander samples and it is suggested that this method could potentially be applied for quality control analyses.