Pouraziz, Parastoo and Yousefi, Milad and Santosa, Adyatma Irawan and Koolivand, Davoud (2023) Genetic Variation between Asian and Mediterranean Populations of Cucurbit Aphid-Borne Yellows Virus. Viruses, 15 (8): 1714. pp. 1-16. ISSN 19994915
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Abstract
Viral symptoms, such as yellowing, leaf deformation, mottling, vein clearing, and reduced yield, were observed in cucurbits in Iran. This study aimed to detect the main suspected causal agent, cucurbit aphid-borne yellows virus (CABYV), in Iran and analyze the genetic diversity among isolates. Two hundred samples were collected from different growing areas between 2019 and 2022. PCR amplification was performed on the P3 and P4 genes. The sequences of 18 Iranian isolates were obtained and deposited in GenBank. Recombination, phylogenetic, and population genetics studies were then carried out for the complete genome and all ORFs sequences, together with other isolates in GenBank. The nucleotide identities of the overlapped ORF3/4 sequences of Iranian isolates were 94.8 to 99.5 among themselves, and with other tested isolates ranging from 94.3 to 99.3. Phylogenetic trees based on the complete genome and the overlapped ORF3/4 showed two major clades, namely Asian and Mediterranean, and the new isolates from Iran were positioned in both clades. The obtained results also suggest that all the genes and two clades of CABYV populations were under negative selection pressure. Furthermore, rare gene flow between these two clades (FST > 0.33) confirmed the high genetic separation among them. © 2023 by the authors.
Item Type: | Article |
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Additional Information: | Cited by: 0; All Open Access, Gold Open Access, Green Open Access |
Subjects: | S Agriculture > S Agriculture (General) |
Depositing User: | Laili Hidayah Hidayah |
Date Deposited: | 26 Aug 2024 06:26 |
Last Modified: | 26 Aug 2024 06:26 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/3178 |