Development of a RT-LAMP assay for real-time detection of criniviruses infecting tomato

Kobayashi, Mayu and Mashiko, Takaaki and Wilisiani, Fariha and Hartono, Sedyo and Nishigawa, Hisashi and Natsuaki, Tomohide and Neriya, Yutaro (2023) Development of a RT-LAMP assay for real-time detection of criniviruses infecting tomato. Journal of Virological Methods, 312: 114662. pp. 1-7. ISSN 01660934

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Abstract

Yellowing symptoms caused by tomato chlorosis virus (ToCV) and tomato infectious chlorosis virus (TICV), both assigned to the genus Crinivirus, resemble nutrient deficiencies. Therefore, early diagnosis of infections will prevent crop damage and the spread of the viruses. In this study, we established a rapid detection method for ToCV and TICV by reverse transcription-loop-mediated isothermal amplification (RT-LAMP). We first designed primer sets for RT-LAMP specific for ToCV and TICV. Next, by selecting the optimum primer set and determining the optimum conditions for the RT-LAMP reaction, each virus was detected within 50 min by piercing the diseased area of a tomato leaf with a toothpick, immersing the toothpick in the reaction solution, and conducting the RT-LAMP reaction. To verify the accuracy of the procedure, 61 tomato leaf samples showing disease symptoms were collected from five regions of Indonesia, and the RT-LAMP results for the samples were identical to those obtained with the commonly used reverse transcription-polymerase chain reaction.

Item Type: Article
Additional Information: Cited by: 3; All Open Access, Hybrid Gold Open Access
Subjects: S Agriculture > S Agriculture (General)
Depositing User: Laili Hidayah Hidayah
Date Deposited: 26 Aug 2024 06:35
Last Modified: 26 Aug 2024 06:35
URI: https://ir.lib.ugm.ac.id/id/eprint/3201

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