Molecular detection of Pepper yellow leaf curl virus, Tomato leaf curl virus, Tomato yellow leaf curl virus, and Mungbean yellow mosaic virus on eggplant, tomato, and pepper at different altitudes in East Java, Indonesia

The genus Begomovirus causes yellow disease is the most damaging and threatening for eggplant, tomato, and pepper production worldwide. Begomoviruses (Family Geminiviridae) are transmitted circulative persistent manner by Bemisia tabaci. This study aims to determine the variation of infection of several species of Begomoviruses in tomato, eggplant, and pepper at different altitudes in East Java. Molecular detection of infection Begomoviruses were discovered using PCR and four pairs of specific primers. Samples were collected at different altitudes in East Java (Kencong, Karangploso, and Pujon). The PCR was successfully applied to detect Pepper yellow leaf curl virus, Tomato leaf curl virus, and Tomato yellow leaf curl virus from tomato, eggplant, and pepper in Kencong. Tomato, eggplant, and pepper from all locations were not infected by Mungbean yellow mosaic virus. Symptoms of Begomovirus infection found in the samples from Kencong were more varied than those found in Karangploso and Pujon. The phylogenetic analysis' findings revealed that the TYLCV Kencong shared the highest homologies with TYLCKaV pepper and eggplant from Indonesia; PepYLCV Kencong shared with PepYLCV Ageratum conyzoides from Indonesia; and ToLCV Kencong shared with ToLCNDV cucumber from Central Java. The molecular detection has shown that there are different combinations of Begomovirus infection at altitudes.