Baseline Susceptibility of the Field Populations of Ostrinia furnacalis in Indonesia to the Proteins Cry1A.105 and Cry2Ab2 of Bacillus thuringiensis

Trisyono, Y. Andi and Aryuwandari, Valentina E. F. and Rahayu, Teguh and Martinelli, Samuel and Head, Graham P. and Parimi, Srinivas and Camacho, Luis R. (2023) Baseline Susceptibility of the Field Populations of Ostrinia furnacalis in Indonesia to the Proteins Cry1A.105 and Cry2Ab2 of Bacillus thuringiensis. Toxins, 15 (10): 602. pp. 1-12. ISSN 20726651

[thumbnail of toxins-15-00602-v2.pdf] Text
toxins-15-00602-v2.pdf - Published Version
Restricted to Registered users only

Download (764kB) | Request a copy

Abstract

Genetically modified MON 89034 corn (Zea mays L.) expressing Bacillus thuringiensis (Bt) insecticidal proteins, viz. Cry1A.105 and Cry2Ab2, is a biotechnological option being considered for the management of the major corn pest in Indonesia, the Asian corn borer (Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)). As a part of a proactive resistance-management program for MON 89034 corn in Indonesia, we assessed the baseline susceptibility of field-collected populations of O. furnacalis to Cry1A.105 and Cry2Ab2 proteins. Dose–response bioassays using the diet-dipping method indicated that the lethal concentration (LC50) values of Cry1A.105 and Cry2Ab2 in 24 different field populations of O. furnacalis ranged from 0.006 to 0.401 µg/mL and from 0.044 to 4.490 µg/mL, respectively, while the LC95 values ranged from 0.069 to 15.233 µg/mL for Cry1A.105 and from 3.320 to 277.584 µg/mL for Cry2Ab2. The relative resistance ratios comparing the most tolerant field populations and an unselected laboratory population were 6.0 for Cry1A.105 and 2.0 for Cry2Ab2 based on their LC50 values. Some field populations were more susceptible to both proteins than the unselected laboratory population. The LC99 and its 95 fiducial limits across the field populations were calculated and proposed as candidate diagnostic concentrations. These data provide a basis for resistance monitoring in Bt Corn and further support building resistance-management strategies in Indonesia.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: Asian corn borer; Cry1A.105; Cry2Ab2; Indonesia; resistance monitoring; susceptibility
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture > Department of Plant Protection
Depositing User: Laili Hidayah Hidayah
Date Deposited: 27 Aug 2024 07:03
Last Modified: 27 Aug 2024 07:03
URI: https://ir.lib.ugm.ac.id/id/eprint/3232

Actions (login required)

View Item
View Item