Genetic diversity of the pathogenic fungus Sarocladium oryzae causing sheath rot on rice using rep-PCR

Pramunadipta, S. and Widiastuti, A. and Wibowo, A. and Priyatmojo, A.Z. (2021) Genetic diversity of the pathogenic fungus Sarocladium oryzae causing sheath rot on rice using rep-PCR. In: The 2nd International Conference on Agriculture and Bio-industry, 27-28 October 2020, Banda Aceh.

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Abstract

Rice becomes the main commodity cultivated in Asia. Sheath rot disease becomes one of the challenges in increasing rice production. Sarocladium oryzae is known to be one of the major pathogens in causing sheath rot disease. This pathogen has been reported in several rice production countries, such as Brazil, India, Taiwan, Philippines, Thailand, including Indonesia, and causing yield loss of up to 85. The study of genetic diversity is important to investigate the rapid shift in genetic structures. A total of six S. oryzae strains showed diverse in molecular using repetitive-PCR (rep-PCR). The isolate SO8 and SO13 isolate SO2 and SO11 and isolate SO3, and SO5 has the relatively same the band patterns in BOX, ERIC, and REP sets of primers. The results of the UPGMA analysis show that S. oryzae in the various primers can be divided into three different group consistency. This result indicates that the rep-PCR method can genetically distinguish S. oryzae. The use of the three main sets, BOX, ERIC, and REP, suggested differentiating the other fungal species. © Published under licence by IOP Publishing Ltd.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Cited by: 4; All Open Access, Gold Open Access
Uncontrolled Keywords: Agriculture; Polymerase chain reaction; Sulfur dioxide; Band patterns; Fungal species; Genetic diversity; Genetic structure; Pathogenic fungi; Philippines; Rapid shifts; Rice production; Biodiversity
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture > Department of Plant Protection
Depositing User: Sri JUNANDI
Date Deposited: 30 Sep 2024 07:09
Last Modified: 30 Sep 2024 07:09
URI: https://ir.lib.ugm.ac.id/id/eprint/4251

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