Expression, Microencapsulation of Recombinant Human Epidermal Growth Factor, and Release Study in Gastric Ulcer Representing Condition

Pratiwi, Riyona Desvy and Yudasari, Nurfina and Rosyidi, Viddy Agustian and Zanjabila, Sabighoh and Abdi Sahid, Muhammad Novrizal and Dewi, Kartika Sari and Fitriagustiani, Fitriagustiani and Fuad, Asrul Muhamad (2021) Expression, Microencapsulation of Recombinant Human Epidermal Growth Factor, and Release Study in Gastric Ulcer Representing Condition. Indonesian Journal of Pharmacy, 32 (4). 493 – 502. ISSN 23389427

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Abstract

Recombinant human epidermal growth factor (rhEGF) has been studied and expressed in various expression systems. It has been also commercialized and clinically used, yet limited to topical diseases. However, being naturally expressed in different tissues, the rhEGF is potential to be applied not only for external wound and skin disorders, but also to regenerates internal damaged epidermal cells such found in gastric ulcer. In the recent study, chitosan microparticles were developed to facilitate delivery of the rhEGF and to overcome gastric degradation that majorly interfere protein, particularly rhEGF oral administration. The rhEGF was expressed in E. coli BL21(DE3) and purified using Ni-NTA chromatography. The refolded rhEGF showed proliferation activity on MCF7 cells. rhEGF loaded chitosan microparticles were stable in the gastric and specifically released the loaded rhEGF in the high oxidative environment in acidic pH representing gastric ulcer condition. © 2021 Universitas Gadjah Mada - Faculty of Pharmacy. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Uncontrolled Keywords: chitosan; recombinant human epidermal growth factor; recombinant protein; unclassified drug; Article; cell proliferation; chromatography; drug delivery system; drug formulation; drug release; Escherichia coli; MCF-7 cell line; microencapsulation; oxidative stress; pH; protein degradation; protein expression; stomach ulcer
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 28 Sep 2024 04:18
Last Modified: 28 Sep 2024 04:18
URI: https://ir.lib.ugm.ac.id/id/eprint/4471

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