Sujono, Tanti Azizah and Nurrochmad, Arief and Lukitaningsih, Endang and Nugroho, Agung Endro (2021) Immunomodulatory effect of petroleum ether extract and ethyl acetate fraction of bengkoang (Pachyrhizus erosus (L.) Urban) in vitro. Indonesian Journal of Pharmacy, 32 (4). 454 – 463. ISSN 23389427
Full text not available from this repository. (Request a copy)Abstract
Bengkoang (Pachyrhizus erosus (L.) Urban) contains phytosterol and the isoflavone daidzein, which are thought to have immunomodulatory activity. There have been no studies reporting on the immunomodulatory effects of bengkoang extract containing polar and semi-polar compounds, such as phytosterols and isoflavone-like compounds. The objective of this study was to evaluate the immunomodulatory effects of bengkoang extracts, including petroleum ether extract (PEE), methanol extract (ME), and the ethyl acetate fraction (EAF) of bengkoang, in vitro. The immunomodulatory effects of PEE, ME, and EAF of bengkoang were determined according to the phagocytic activity of macrophages based on phagocytosis of latex beads, lymphocyte proliferation, and detection of cytokine production of tumor necrosis factor-α (TNF-α) interleukin-6 (IL-6), and interleukin-10 (IL-10) levels. Results: The phagocytic index and phagocytic capacity of ME, PEE, and EAF of bengkoang on macrophage cells were significantly increased (p < 0.05), whereas on lymphocyte proliferation, ME showed a trend of increasing activity (p < 0.05). On the other hand, the PEE effect showed a decreasing trend of activity, and the others had no significant effect on lymphocyte proliferation (p > 0.05), and ME of bengkoang enhanced the levels of the cytokines TNF-α and IL-6. In contrast, PEE and EAF of bengkoang decreased TNF-α and IL-6 levels compared with the control group. All of the bengkoang extracts decrease the production of the anti-inflammatory cytokine IL-10. In conclusion, this study showed that PEE, ME, and EAF of bengkoang could increase the non-specific immune response (phagocytic activity) but had a lesser effect on the specific immune response (lymphocyte proliferation). The ME of bengkoang acts as an immunostimulant by increasing the levels of the inflammatory cytokines TNF-α and IL-6 and decreasing those of the anti-inflammatory cytokine IL-10. Further investigation of in vivo immunomodulatory effects is needed to determine its effect on systemically innate and adaptive immune responses. © 2021 Universitas Gadjah Mada - Faculty of Pharmacy. All rights reserved.
Item Type: | Article |
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Additional Information: | Cited by: 0; All Open Access, Gold Open Access |
Uncontrolled Keywords: | acetic acid ethyl ester; immunomodulating agent; interleukin 10; interleukin 6; methanol; Pachyrhizus erosus extract; petroleum ether; plant extract; tumor necrosis factor; unclassified drug; adaptive immunity; animal cell; animal experiment; animal tissue; Article; cell phagocytosis; controlled study; cytokine production; immunomodulation; in vitro study; lymphocyte proliferation; macrophage; male; mouse; nonhuman; Pachyrhizus erosus |
Subjects: | R Medicine > RS Pharmacy and materia medica |
Divisions: | Faculty of Pharmacy |
Depositing User: | Sri JUNANDI |
Date Deposited: | 27 Sep 2024 06:41 |
Last Modified: | 27 Sep 2024 06:41 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/4528 |