Genistein affects estrogen receptor alpha (ER-α)/estrogen receptor beta (ER-β) ratio, and nuclear factor-Kappa beta (NF-κβ) in mice model of endometriosis

Sutrisno, Sutrisno and Gayatri, Mergy and Wiyasa, I Wayan Arsana and Kalsum, Umi and Andarini, Sri (2021) Genistein affects estrogen receptor alpha (ER-α)/estrogen receptor beta (ER-β) ratio, and nuclear factor-Kappa beta (NF-κβ) in mice model of endometriosis. Bahrain Medical Bulletin, 43 (3). 601 – 608. ISSN 10128298

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Abstract

Background: Currently, treatment of endometriosis remains expensive. One of low-cost treatments for this disease is genistein. This study aimed to assess the effect of genistein on Estrogen Receptor Alpha (ER-α) and Estrogen Receptor Beta (ER-β) ratio and Nuclear Factor-Kappa Beta (NF-κβ) in mice model of endometriosis remain unclear. Methods: This study is experimental using post-test research design. Twenty-four female mice (Mus musculus) model of endometriosis were fed genistein. The mice were divided into four groups with various doses of genistein, i.e., 1,30 mg/mice/day (group P1); 1,95 mg/mice/day (group P2); 2,60 mg/mice/day (P3), and; 3,25 mg/mouse/ day (group P4). We fed the mouse using a sonde for 14 days. On the fifth day, the mice were sacrificed and dissected to take the peritoneal tissue. The tissue was coloured using Immunohistochemistry staining (IH) and microscopically assessed at 400x magnification calculated at 1000 cells in order to observe the expression of RE-α, RE-β, and NFkB. Results: Genistein at various doses had a significant effect on the RE-α / RE-β ratio. The higher the genistein dose given, the lower the RE-α / RE-β ratio. In addition, genistein dose of 3.25 mg / day significantly reduced NFkB expression. Conclusions: Genistein in various doses have been shown to significantly influence the RE-α / RE-β ratio. In other words, the higher the dose of genistein given will decrease the RE-α / RE-β ratio. © 2021, Bahrain Medical Bulletin. All rights reserved.

Item Type: Article
Additional Information: Cited by: 1
Uncontrolled Keywords: estrogen receptor alpha; estrogen receptor beta; genistein; immunoglobulin enhancer binding protein; animal experiment; animal model; animal tissue; apoptosis; Article; cell proliferation; controlled study; endometriosis; endometrium cancer; endometrium cell; female; gene expression; histopathology; immunohistochemistry; immunoreactivity; mouse; mouse model; Mus musculus; nonhuman; peritoneal tissue; protein expression
Subjects: R Medicine > RB Biomedical Sciences
Divisions: Faculty of Medicine, Public Health and Nursing > Biomedical Sciences
Depositing User: Sri JUNANDI
Date Deposited: 27 Sep 2024 03:41
Last Modified: 27 Sep 2024 03:41
URI: https://ir.lib.ugm.ac.id/id/eprint/4558

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