Novitasari, Dhania and Jenie, Riris Istighfari and Wulandari, Febri and Utomo, Rohmad Yudi and Putri, Dyaningtyas Dewi Pamungkas and Kato, Jun-Ya and Meiyanto, Edy (2021) Curcumin-like structure (Cca-1.1) induces permanent mitotic arrest (senescence) on triple-negative breast cancer (tnbc) cells, 4t1. Research Journal of Pharmacy and Technology, 14 (8). 4375 – 4382. ISSN 09743618
Full text not available from this repository. (Request a copy)Abstract
Triple-negative breast cancer (TNBC) remains as the deadliest cancer type due to the lack of treatment options. Hence, several attempts have been made to develop new anticancer for TNBC therapy. This study intended to challenge curcumin analog (CCA)-1.1, which is derived from pentagamavunone-1 structure, against the 4T1 cell line and TNBC cell model, covering the cytotoxic activity in correlation with cell cycle progression, apoptosis induction, reactive oxygen species (ROS) generation, and senescence evidence. The cell viability, cell cycle profile, apoptosis induction, intracellular ROS level, and senescence induction were determined in vitro using trypan blue exclusion, propidium iodide (PI) staining, Annexin-PI staining, dichlorofluorescein diacetate staining, and senescence-associated-β-gal method. CCA-1.1 showed cytotoxic activity on 4T1 cells, giving half maximal inhibitory concentration value of 3μM, but was less toxic on non-cancerous 3T3-L1 cells. CCA-1.1 induced rapid cell death and inhibited cell cycle progression at the mitotic phase. Instead, of causing apoptosis, CCA-1.1 induced mitotic catastrophe. Furthermore, CCA-1.1 itself increased the intracellular ROS level and induced senescence, possibly through catastrophic cell death. Altogether, our preliminary study strengthens the potency of CCA-1.1 for its anticancer activities against TNBC cells and prospective to be pharmaceutically developed as a novel candidate for cancer therapy. © RJPT All right reserved.
Item Type: | Article |
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Additional Information: | Cited by: 13 |
Uncontrolled Keywords: | curcumin; cyclin E; dichlorofluorescein; propidium iodide; protein bcl 2; protein p53; reactive oxygen metabolite; steroid; 3T3-L1 cell line; 4T1 cell line; animal cell; animal experiment; animal model; antineoplastic activity; apoptosis; apoptosis assay; Article; cancer therapy; cell cycle arrest; cell cycle G1 phase; cell cycle progression; cell cycle regulation; cell death; cell viability; controlled study; cytotoxicity; disaster; DNA fragmentation; DNA replication; flow cytometry; G2 phase cell cycle checkpoint; IC50; mitochondrial membrane potential; mitosis inhibition; molecular docking; mouse; MTT assay; nonhuman; oxidative stress; propidium iodide assay; protein expression; senescence; triple negative breast cancer; trophoblast; trypan blue assay |
Subjects: | R Medicine > RS Pharmacy and materia medica |
Divisions: | Faculty of Pharmacy |
Depositing User: | Sri JUNANDI |
Date Deposited: | 27 Sep 2024 00:58 |
Last Modified: | 27 Sep 2024 00:58 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/4593 |