CCA-1.1, a Novel Curcumin Analog, Exerts Cytotoxic anti-Migratory Activity toward TNBC and HER2-Enriched Breast Cancer Cells

Novitasari, Dhania and Jenie, Riris Istighfari and Utomo, Rohmad Yudi and Kato, Jun-Ya and Meiyanto, Edy (2021) CCA-1.1, a Novel Curcumin Analog, Exerts Cytotoxic anti-Migratory Activity toward TNBC and HER2-Enriched Breast Cancer Cells. Asian Pacific Journal of Cancer Prevention, 22 (6). 1827 – 1836. ISSN 15137368

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Abstract

Objective: Chemoprevention curcumin Analog-1.1 (CCA-1.1) demonstrates antineoplastic effect toward cancer cells. By using triple-negative breast cancer (TNBC), 4T1, and human epidermal growth factor receptor 2 (HER2)-enriched metastatic cells (MCF-7/HER2), we evaluate the cytotoxic and antimigration activities from CCA-1.1. Methods: The cytotoxic activities from a single treatment of CCA-1.1 and in combination with doxorubicin were determined through MTT assay. We also calculated the selectivity index and combination index of CCA-1.1 from the cytotoxic data. Migrating cells were evaluated using wound healing assay, and the MMP2 and MMP9 secretion levels were determined through gelatin zymography. Results: As hypothesized, CCA-1.1 performed cytotoxic activity during treatment in 4T1 and MCF-7/HER2 cancer cells with good selectivity (Selectivity Index >2). In addition, CCA-1.1 demonstrated a synergistic effect in combinatorial treatment with a low dose of doxorubicin. A single treatment of CCA-1.1 repressed cell migration in 4T1 and MCF-7/HER2 cells. Under gelatin zymography, CCA-1.1 subsided the activities of MMP-9, thereby revealing the potency of CCA-1.1 as an anti-migratory agent. Moreover, MMP-9 was also eminently expressed in TNBC and HER2-enriched breast cancer cells when compared with that in other subtypes. Conclusion: Our preliminary study collectively reinforces the potential effect of CCA-1.1 through the inhibition of highly aggressive cell migration, particularly in breast cancer. © 2021. All Rights Reserved.

Item Type: Article
Additional Information: Cited by: 14; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: Antineoplastic Agents; Cell Movement; Cell Proliferation; Chemoprevention; Curcumin; Doxorubicin; Drug Resistance, Neoplasm; Drug Synergism; Female; Humans; Receptor, ErbB-2; Triple Negative Breast Neoplasms; Tumor Cells, Cultured; 2- ((4-hydroxy-3,5-dimethylphenyl)methylidene)-5-((3-methoxy-4,5-dimethylphenyl)methylidene)cyclopentan-1-one; antineoplastic agent; curcumin; doxorubicin; epidermal growth factor receptor 2; ERBB2 protein, human; cell motion; cell proliferation; chemoprophylaxis; drug effect; drug potentiation; drug resistance; female; human; triple negative breast cancer; tumor cell culture
Subjects: R Medicine > RB Biomedical Sciences
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 25 Sep 2024 07:35
Last Modified: 25 Sep 2024 07:35
URI: https://ir.lib.ugm.ac.id/id/eprint/4637

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