Setyawati, Tri and Kusuma, Rio Jati and Luglio, Harry Freitag and Oktiyani, Neni and Sunarti, Sunarti and Nur, Rosmala and Hendra, Syaiful (2021) The effect of gembili starch (Dioscorea esculenta) and eubacterium rectal supplementation on skeletal muscle peroxisome proliferator-activated receptor γ coactivator 1α (pgc-1α) expression in diabetic mice models. Open Access Macedonian Journal of Medical Sciences, 9. 1061 – 1067. ISSN 18579655
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BACKGROUND: Gembili or Dioscorea esculenta is a local food that is produced by several areas in Indonesia. Few studies have reported its health benefits for diabetes mellitus but a little is understood about its mechanism of action. PGC-1α is a transcriptional coactivator for genes that involved in energy metabolism and increased expression of this gene has previously been associated with improved insulin sensitivity. AIM: The objective of this study was to investigate the effect of Gembili starch and Gembili starch with butirogenic bacteria Eubacterium rectal on PGC-1α expression in skeletal muscle of diabetic mice. MATERIALS AND METHODS: Three months old male diabetic Wistar mice were divided into groups based on dietary supplement: Gembili starch only; Gembili starch with E. rectal; and E. rectal only. Positive (diabetic mice) and negative (non-diabetic) control groups were used in this study. After 4 weeks of supplementation, mice were sacrificed and muscle tissue was taken from musculus vastus latissimus. Plasma blood glucose was measured before and after intervention. PGC-1α expression was measured with immunohistochemistry and quantified by dividing cells that produce PGC-1α with total cells. RESULTS: Plasma blood glucose was reduced after invention in group that received Gembili starch only (p < 0.001); Gembili starch with E. rectal (p < 0.001); and E. rectal only (p < 0.001). The protein expression of PGC-1α in diabetic mice receiving Gembili starch only was significantly higher compared to control (p < 0.05). CONCLUSION: This study shown that Gembili starch supplementation was able to improve glucose control in diabetic mice and this effect was obtained perhaps through PGC-1α activation. Further study is needed to investigate the effect of Gembili starch supplementation on fat metabolism. © 2021 Tri Setyawati, Rio Jati Kusuma, Harry Freitag Luglio, Neni Oktiyani, Sunarti Sunarti, Rosmala Nur, Syaiful Hendra.
Item Type: | Article |
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Additional Information: | Cited by: 0; All Open Access, Gold Open Access |
Uncontrolled Keywords: | acetic acid; alcohol; Dioscorea extract; formaldehyde; glucose; insulin; liquid paraffin; peroxisome proliferator activated receptor gamma coactivator 1alpha; propionic acid; starch; streptozocin; xylene; animal experiment; animal model; animal tissue; Article; blood glucose monitoring; blood sampling; centrifugation; colony forming unit; controlled study; dehydration; diabetes mellitus; dietary supplement; Dioscorea esculenta; energy metabolism; enzyme linked immunosorbent assay; Eubacterium; fasting; freeze drying; gene expression; glucose blood level; glucose tolerance test; homeostasis model assessment; immunohistochemistry; incubation time; insulin blood level; insulin sensitivity; light dark cycle; lipid metabolism; male; mouse; muscle tissue; non insulin dependent diabetes mellitus; nonhuman; skeletal muscle; spectrophotometry; vastus lateralis muscle |
Subjects: | R Medicine > RB Biomedical Sciences |
Divisions: | Faculty of Medicine, Public Health and Nursing > Public Health and Nutrition |
Depositing User: | Sri JUNANDI |
Date Deposited: | 23 Sep 2024 03:57 |
Last Modified: | 23 Sep 2024 03:57 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/4763 |