Satuman, Satuman and Sari, Desi Sandra and Rachmi, Eva and Tanggo, Eddy Herman and Notobroto, Hari Basuki and Sudiana, Ketut and Mubarika, Sofia and Rantam, Fedik Abdul and Soemarno, Soemarno and Warsito, Eddy Bagus (2021) The effect of acute and chronic infection-induced byavraprotein of salmonella typhimurium on radical oxygen species, phosphatase and tensin homolog, and cellular homolog expression during the development of colon cancer. Open Access Macedonian Journal of Medical Sciences, 9 (A). 343 – 351. ISSN 18579655
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Abstract
AIM: The study aimed to analyze the effect of AvrA effector protein of Salmonella typhimurium in inducing colon cancer through increased of radical oxygen species (ROS), phosphatase and tensin homolog (PTEN), and avian myelocytomatosis virus oncogene cellular homolog (c-Myc) expression, in mice model of colorectal cancer. METHODS: This study used Balb/c mice which were divided into four types of groups: Negative control, exposed to azoxymethane (AOM), treatment with AOM, and AvrA (AOM+AvrA), and treatment with AOM and S. typhimurium (AOM + S. typhimurium). Each type consists of a 1-week treatment group and a 12-weeks treatment group, with a final number of eight groups. S. typhimurium-specific protein (AvrA) was isolated and then injected to AOM + AvrA groups (40 μg/50 μl), intraperitoneally. S. typhimurium was administered orally to AOM + S. typhimurium groups. ROS production in peripheral blood mononuclear cells was measured by flow cytometry. PTEN and c-Myc expression in colon tissue were detected through immunohistochemistry. RESULTS: The study showed that ROS production was higher in the 12-week AOM + S. typhimurium treatment group compared with other 12-week treatment groups (p < 0.05). AOM + AvrA and AOM + S. typhimurium groups demonstrated a decrease of PTEN expression and an increase of c-Myc expression in colon tissue, compared to AOM groups, both in 1-week and 12-weeks treatment (p < 0.05). CONCLUSION: AvrA effector protein from S. typhimurium increased ROS production and c-Myc expression while suppressed PTEN expression as markers of colorectal cancer, both in acute and chronic infections. © 2021 Satuman Satuman, Desi Sandra Sari, Eva Rachmi, Eddy Herman Tanggo, Hari Basuki Notobroto, Ketut Sudiana, Sofia Mubarika, Fedik Abdul Rantam, Soemarno Soemarno, Eddy Bagus Warsito.
Item Type: | Article |
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Additional Information: | Cited by: 3; All Open Access, Gold Open Access |
Uncontrolled Keywords: | AvrA protein; azoxymethane; bacterial protein; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; reactive oxygen metabolite; unclassified drug; animal cell; animal experiment; animal model; animal tissue; Article; Bagg albino mouse; chronic infection; colorectal cancer; controlled study; enzyme linked immunosorbent assay; flow cytometry; immunohistochemistry; mouse; myeloid leukemia; nonhuman; oncogene c myc; peripheral blood mononuclear cell; Salmonella enterica serovar Typhimurium |
Subjects: | R Medicine > RB Pathology |
Divisions: | Faculty of Medicine, Public Health and Nursing > Public Health and Nutrition |
Depositing User: | Sri JUNANDI |
Date Deposited: | 18 Sep 2024 02:13 |
Last Modified: | 18 Sep 2024 02:13 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/4853 |