A potential zoonotic parasite: Cryptosporidium parvum transmission in rats, pigs and humans in west lombok, indonesia.

Resnhaleksmana, Ersandhi and Wijayanti, Mahardika Agus and Artama, Wayan Tunas (2021) A potential zoonotic parasite: Cryptosporidium parvum transmission in rats, pigs and humans in west lombok, indonesia. Heart International, 15 (2). 44 – 51. ISSN 18261868

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Abstract

Background: Cryptosporidium is a neglected zoonotic disease, but with the expansion of the human community into the animal environment, its incidence is increasing. Animals such as rats and pigs can act as intermediate hosts and transmit Cryptosporidium to humans due to their proximity. Transmission occurs due to the ability of Cryptosporidium to survive in any new host. The research aimed to identify and describe the transmission of Cryptosporidium from animals to humans. Materials and Methods: This research was a cross sectional study and samples were collected from 84 rats caught in residential areas, 205 pigs, and 438 humans in West Lombok. Fecal samples were examined using polymerase chain reaction (PCR) and sequencing to isolate the presence of Cryptosporidium, and identify the genetic similarity of the parasites found in rats and pigs with those that infect humans. Results: The PCR results found Cryptosporidium parvum in 4.76 (4/84) in rats; 6.34 13/205) in pigs; and 0.91 (4/438) in humans. The sequencing results showed genetic kinship of C. parvum in rats, pigs, and humans. Based on sequence confirmation from Gene Banks and edited using ClustalW with MEGA X software, there are genetic similarities between Cryptosporidium isolates from West Lombok and C. suis isolates of cattle from Uganda and C. suis isolates of pigs from Slovakia. Conclusion: There are genetic similarities of Cryptosporidium in animals and humans, requiring that the Public Health programs in those contaminated areas must receive priority attention to prevent further transmission of these potentially fatal parasites. © 2021, Touch Medical Media. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Green Open Access, Hybrid Gold Open Access
Uncontrolled Keywords: animal experiment; animal tissue; Article; bacterium identification; bacterium isolation; comparative study; cross-sectional study; cryptosporidiosis; Cryptosporidium; Cryptosporidium parvum; disease transmission; DNA extraction; DNA isolation; DNA sequence; gene amplification; gene detection; gene sequence; genetic analysis; genetic polymorphism; genetic similarity; genotype; geographic distribution; Giardia intestinalis; haplotype; high throughput sequencing; human; Indonesia; molecular epidemiology; nonhuman; phylogenetic tree; pig; polymerase chain reaction; risk factor; sequence alignment; sequence analysis; zoonosis; zoonotic transmission
Subjects: R Medicine > RB Biomedical Sciences
Divisions: Faculty of Medicine, Public Health and Nursing > Public Health and Nutrition
Depositing User: Sri JUNANDI
Date Deposited: 17 Sep 2024 06:53
Last Modified: 17 Sep 2024 06:53
URI: https://ir.lib.ugm.ac.id/id/eprint/4865

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