Satria, Rahmat Dani and Huang, Tzu-Wen and Jhan, Ming-Kai and Shen, Ting-Jing and Tseng, Po-Chun and Wang, Yun-Ting and Yang, Zhen-Yu and Hsing, Chung-Hsi and Lin, Chiou-Feng (2021) Increased TNF- α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus. Journal of Immunology Research, 2021. ISSN 23148861
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Abstract
During the acute febrile phase of dengue virus (DENV) infection, viremia can cause severe systemic immune responses accompanied by hematologic disorders. This study investigated the potential induction and mechanism of the cytopathic effects of DENV on peripheral blood cells ex vivo. At one day postinfection, there was viral nonstructural protein NS1 but no further virus replication measured in the whole blood culture. Notably, DENV exposure caused significant vacuolization in monocytic phagocytes. With a minor change in the complete blood cell count, except for a minor increase in neutrophils and a significant decrease in monocytes, the immune profiling assay identified several changes, particularly a significant reduction in CD14-positive monocytes as well as CD11c-positive dendritic cells. Abnormal production of TNF-α was highly associated with the induction of vacuolization. Manipulating TNF-α expression resulted in cytopathogenic effects. These results demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α. © 2021 Rahmat Dani Satria et al.
Item Type: | Article |
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Additional Information: | Cited by: 6; All Open Access, Gold Open Access |
Uncontrolled Keywords: | Aedes; Animals; Blood Cell Count; Cell Line; Coculture Techniques; Cricetinae; Dengue; Dengue Virus; Healthy Volunteers; Humans; Monocytes; Primary Cell Culture; Systemic Inflammatory Response Syndrome; Tumor Necrosis Factor-alpha; Viremia; CD14 antigen; CD16 antigen; CD19 antigen; CD4 antigen; CD56 antigen; CD8 antigen; glycoprotein p 15095; HLA DR antigen; interleukin 2 receptor alpha; L selectin; nonstructural protein 1; tumor necrosis factor; TNF protein, human; tumor necrosis factor; antigen detection; Article; blood cell count; blood culture; blood sampling; cell vacuole; coculture; controlled study; dendritic cell; dengue; Dengue virus; enzyme linked immunosorbent assay; ex vivo study; Giemsa stain; human; human cell; immune response; monocyte; nonhuman; phagocyte; protein expression; virus culture; Aedes; animal; blood; cell line; coculture; complication; dengue; Dengue virus; hamster; immunology; metabolism; normal human; pathology; primary cell culture; systemic inflammatory response syndrome; viremia; virology |
Subjects: | R Medicine > RB Biomedical Sciences |
Divisions: | Faculty of Medicine, Public Health and Nursing > Public Health and Nutrition |
Depositing User: | Sri JUNANDI |
Date Deposited: | 12 Sep 2024 07:20 |
Last Modified: | 12 Sep 2024 07:20 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/4924 |