Association between three variants in the prkaa2 gene, rs2796498, rs9803799, and rs2746342, with 10-year ascvd risk on newly diagnosed t2dm in Yogyakarta, Indonesia

Virginia, Dita Maria and Wahyuningsih, Mae Sri Hartati and Nugrahaningsih, Dwi Aris Agung (2021) Association between three variants in the prkaa2 gene, rs2796498, rs9803799, and rs2746342, with 10-year ascvd risk on newly diagnosed t2dm in Yogyakarta, Indonesia. Open Access Macedonian Journal of Medical Sciences, 9 (A). 541 – 547. ISSN 18579655

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Abstract

BACKGROUND: AMPK has pivotal roles in glucose and lipid metabolism, including AMPKα2, which PRKAA2 encodes. Metformin as an anti-hyperglycemia agent acts through AMPK. Poor glycemic control among patients with type 2 diabetes mellitus (T2DM) could increase atherosclerosis cardiovascular disease (ASCVD) risk. Therefore, PRKAA2 genetic variation might contribute to 10-year ASCVD risk in patients with newly diagnosed T2DM receiving monotherapy metformin. AIM: The study aimed to detect an association between PRKAA2 genetic variation with 10 year-ASCVD risk among newly diagnosed T2DM patients prescribed monotherapy metformin. METHODS: This present study was a casecontrol study involving 107 participants. Analysis of PRKAA2 genetic variation was performed using the TaqMan assay. RESULTS: A total of 91 participants who fulfilled our criteria enrolled in this study. Most of the participants were female, with a mean age of 54.40 ± 7.75 years old, mean HbA1c level of 8.35 ± 1.31 and the lipid profile indicated normal conditions. There was a significant difference in age (p < 0.01), HbA1c level (p = 0.04), sex (p < 0.01), and smoking status (p < 0.01) between low-risk and high-risk groups. The GT genotype of rs9803799 had 187.86 times higher possibility for high-risk of 10-year ASCVD risk than TT genotype (OR = 187.86, 95CI:2.98–11863.51). The dominant model of rs9803799 showed that GT+GG had a 94.33 times higher possibility for high-risk of 10-year ASCVD risk than TT genotype (OR=94.33; 95CI:2.32–3841.21). Other results showed that the G allele of rs980377 had 20.48 times higher possibility for high-risk of 10-year ASCVD risk than the T allele (OR = 20.48; 95CI:1.48–283.30). These associations were found after multivariate analysis. CONCLUSION: Our findings indicated that rs9803799 as one of the PRKAA2 genetic variations might impact the 10-year ASCVD risk among newly diagnosed T2DM patients receiving monotherapy metformin. After considering non-genetic factors, patient assessment should include potential genetic factors in cases with hyperglycemia involving treatment affecting glucose and lipid metabolism such as monotherapy metformin. © 2021 Dita Maria Virginia, Mae Sri Hartati Wahyuningsih, Dwi Aris Agung Nugrahaningsih.

Item Type: Article
Additional Information: Cited by: 2; All Open Access, Gold Open Access
Uncontrolled Keywords: acetylsalicylic acid; cholesterol; cyclic AMP dependent protein kinase catalytic subunit; edetic acid; genomic DNA; glucose; hemoglobin A1c; high density lipoprotein cholesterol; hydroxymethylglutaryl coenzyme A reductase inhibitor; hydroxymethylglutaryl coenzyme A reductase kinase; low density lipoprotein cholesterol; metformin; triacylglycerol; adult; aged; anthropometry; Article; blood sampling; carotid atherosclerosis; case control study; cholesterol blood level; controlled study; diabetes mellitus; diastolic blood pressure; DNA isolation; fasting; female; genetic variation; genotype; glucose blood level; glycemic control; high density lipoprotein cholesterol level; high performance liquid chromatography; human; hyperglycemia; hypertension; interview; lipid fingerprinting; lipid metabolism; low density lipoprotein cholesterol level; major clinical study; male; medical record; non insulin dependent diabetes mellitus; obesity; people by smoking status; physical activity; questionnaire; systolic blood pressure; triacylglycerol blood level; waist circumference
Subjects: R Medicine > RB Biomedical Sciences
Divisions: Faculty of Medicine, Public Health and Nursing > Biomedical Sciences
Depositing User: Sri JUNANDI
Date Deposited: 12 Sep 2024 07:25
Last Modified: 12 Sep 2024 07:25
URI: https://ir.lib.ugm.ac.id/id/eprint/4925

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