Harahap, I.A. and Mariyatun, M. and Hasan, P.N. and Pamungkaningtyas, F.H. and Widada, J. and Utami, T. and Cahyanto, M.N. and Juffrie, M. and Dinoto, A. and Nurfiani, S. and Zulaichah, E. and Sujaya, I.N. and Rahayu, E.S. (2021) Recovery of Indigenous probiotic Lactobacillus plantarum Mut-7 on healthy Indonesian adults after consumption of fermented milk containing these bacteria. Journal of Food Science and Technology, 58 (9). 3525 – 3532. ISSN 00221155
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Abstract
Probiotics are live microorganisms that confer health benefits on the host when administered in adequate amounts, such as to support the balance of gut microbiota. In this study, the selected indigenous probiotic strain, Lactobacillus plantarum Mut-7, was used as a starter culture to produce fermented milk. A total of 28 healthy Indonesian youngsters and adults (male/female: 16/12; age 19.4–22.9 years old; normal BMI range 18.6–22.7 kg/m2) were supplemented with the fermented milk. This study aimed to determine the recovery of L. plantarum Mut-7 through molecular analysis from the subjects feces after ingestion of 140 mL fermented milk containing 7.0 log10 CFU/ml of L. plantarum Mut-7 for 15 days. Molecular detection was performed using the rep-PCR technique and sequencing of DNA 16S rRNA. Consumption of fermented milk containing L. plantarum Mut-7 enabled reduction of total E. coli and Coliform non-E. coli in several subjects. It was able to increase the total LAB and total L. plantarum in subjects’ feces. The number of L. plantarum and mesophilic LAB increased by 5.5 ± 1.6 log10 CFU/g, 1.8 ± 0.8 log10. On the other side, thermophilic LAB increased by 2.8 ± 3.0 log10 CFU/g in 23 out of 28 subjects. These findings proved that L. plantarum survived in the human gastrointestinal tract. Based on the molecular identification technique using rep-PCR technique and sequencing of gene 16S rRNA, two isolates had similarity to L. plantarum Mut-7 by a coefficient value of 100. © 2021, Association of Food Scientists & Technologists (India).
Item Type: | Article |
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Additional Information: | Cited by: 2; All Open Access, Green Open Access |
Uncontrolled Keywords: | Bacilli; Dairies; Escherichia coli; Gene encoding; Polymerase chain reaction; Probiotics; RNA; Gastrointestinal tract; Lactobacillus plantarum; Molecular analysis; Molecular detection; Molecular identification; Probiotic lactobacilli; Probiotic strain; Starter cultures; Fermented milk |
Subjects: | T Technology > TX Home economics T Technology > TX Home economics > Nutrition. Foods and food supply |
Divisions: | Faculty of Agricultural Technology > Food and Agricultural Product Technology |
Depositing User: | Sri JUNANDI |
Date Deposited: | 25 Oct 2024 03:00 |
Last Modified: | 25 Oct 2024 03:00 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/5546 |