In vitro anthelmintic activity of clove-leaf extract (Syzygium aromaticum) against Ascaridia galli

Parasitic worms that live and develop in the gastrointestinal tract are often found in poultry. Ascaridia galli is the most common type of these worms. Commercial anthelmintic drugs cause many problems including environmental pollution, adverse effects on host health, and widespread anthelmintic resistance. As an alternative, clove leaves are very valuable as an anthelmintic because they contain eugenols, tannins, saponins, flavonoids. This study aims to determine the anthelmintic potential of the clove-leaf ethanol extract (CLE) against Ascaridia galli. A total of 240 worms were divided into 8 groups to be treated with CLE at seven different concentrations (20, 40, 60, 80, 100, 120, and 140 mg/mL) and saline as control, respectively with 3 repetitions. Ten worms of each group submerged into 25ml of the test solution for 3-9 hours, then evaluated using a Scanning Electron Microscope (SEM). The results showed the highest mean mortality at 3h, 6h, and 9h observation was CLE 140 mg/mL. The ethanol extract of clove leaves caused surface changes resulting in damages to the cuticle of A. galli adult worm. It can be concluded that CLE possesses anthelmintic activity. © 2021 Fundacion CIPAV. All rights reserved.