Process for an efficient lentiviral cell transduction

Pirona, Anna Chiara and Oktriani, Risky and Boettcher, Michael and Hoheisel, Jörg D. (2021) Process for an efficient lentiviral cell transduction. Biology Methods and Protocols, 5 (1). 1 – 8. ISSN 23968923

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Abstract

The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: lentivirus, cell transduction, CRISPR-Cas, shRNA
Subjects: R Medicine > RP Public Health and Nutrition
R Medicine > RT Nursing
Divisions: Faculty of Medicine, Public Health and Nursing > Nursing
Depositing User: Sri JUNANDI
Date Deposited: 30 Oct 2024 03:47
Last Modified: 30 Oct 2024 03:47
URI: https://ir.lib.ugm.ac.id/id/eprint/5948

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