Pirona, Anna Chiara and Oktriani, Risky and Boettcher, Michael and Hoheisel, Jörg D. (2021) Process for an efficient lentiviral cell transduction. Biology Methods and Protocols, 5 (1). 1 – 8. ISSN 23968923
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Abstract
The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.
Item Type: | Article |
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Additional Information: | Library Dosen |
Uncontrolled Keywords: | lentivirus, cell transduction, CRISPR-Cas, shRNA |
Subjects: | R Medicine > RP Public Health and Nutrition R Medicine > RT Nursing |
Divisions: | Faculty of Medicine, Public Health and Nursing > Nursing |
Depositing User: | Sri JUNANDI |
Date Deposited: | 30 Oct 2024 03:47 |
Last Modified: | 30 Oct 2024 03:47 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/5948 |