Correlation Analyses of the Oral Biofilm Growth Inhibition towards Hydrophobicity Reduction of Oral Pathogenic Bacteria

Utami, Diyah T. and Hertiani, Triana and Pratiwi, Sylvia U. T. and Haniastuti, Tetiana and Randy, Ahmad and Priyanto, Jepri A. and Prastya, Muhammad E. (2023) Correlation Analyses of the Oral Biofilm Growth Inhibition towards Hydrophobicity Reduction of Oral Pathogenic Bacteria. Tropical Journal of Natural Product Research, 7 (10). 4141 – 4145. ISSN 26160684

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Abstract

Bacteria have a tendency to form multicellular biofilms, which adhere to surfaces and contain extracellular polymeric substances (EPS). This tendency is accompanied by hydrophobic interactions, which are vital in the biofilm attachment process. Oral biofilms contribute to a range of oral health issues, including gingivitis, dental caries, and halitosis. Therefore, this research aimed to investigate the correlation between oral biofilm growth inhibition and hydrophobicity reduction in Streptococcus mutants, Streptococcus sanguinis, Lactobacillus acidophilus, and Actinomyces viscosus. Essential oil-derived compounds, namely eugenol, C-10 massoia lactone, thymol, cinnamaldehyde, and zerumbone, were applied in the experiment. A microdilution assay using crystal violet staining evaluated the oral microbes' biofilm growth. The adhesion of microbes to hexadecane was measured to assess hydrophobicity reduction, which was analyzed correlatively using PAST (Paleontological Statistics) software and the Principal Component Analysis (PCA) method. The results showed a positive correlation of the hydrophobicity reduction towards the biofilm formation inhibition for all tested microbes (graphical angle <45°). The Principal Component (PC) analysis, based on the eigene values, showed that PC1 and PC2 accounted for 54.149 and 25.652 of the total variation, respectively. These two components explained 79.801 of the total variation, indicating a significant level of variability. This finding supported the notion that a greater reduction in microbial hydrophobicity was associated with stronger inhibitory activity against planktonic growth. The hydrophobicity reduction assay may indicate a potential of bioactive compounds against biofilm growth inhibition of oral microbes. © 2023 Utami et al.

Item Type: Article
Additional Information: Cited by: 1; All Open Access, Hybrid Gold Open Access
Uncontrolled Keywords: cinnamaldehyde; essential oil; eugenol; hexadecane; lactone; thymol; zerumbone; Actinomyces viscosus; antibacterial activity; antibiofilm activity; Article; bacterium adherence; biofilm; biofilm matrix; colony formation; correlation analysis; dental caries; gingivitis; growth inhibition; halitosis; health; hydrophobicity; Lactobacillus acidophilus; minimum inhibitory concentration; mouth flora; mouth hygiene; nonhuman; Oral Biofilm Growth Inhibition; Oral Pathogenic Bacteria; physical chemistry; principal component analysis; spectrophotometry; Streptococcus mutans; Streptococcus sanguinis; surface tension
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 30 Oct 2024 07:34
Last Modified: 30 Oct 2024 07:34
URI: https://ir.lib.ugm.ac.id/id/eprint/5996

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