Tumour-suppressive effects of curcumin analogs CCA-1.1 and Pentagamavunone-1 in colon cancer: In vivo and in vitro studies

Wulandari, Febri and Ikawati, Muthi and Widyarini, Sitarina and Kirihata, Mitsunori and Novitasari, Dhania and Kato, Jun-Ya and Meiyanto, Edy (2023) Tumour-suppressive effects of curcumin analogs CCA-1.1 and Pentagamavunone-1 in colon cancer: In vivo and in vitro studies. Journal of Advanced Pharmaceutical Technology and Research, 14 (4). 317 – 324. ISSN 22314040

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Abstract

This study aimed to evaluate the efficacy of Chemoprevention Curcumin Analog-1.1 (CCA-1.1) and Pentagamavunone-1 (PGV-1) in vivo and in vitro in colorectal cancer model. CCA-1.1 or PGV-1 was administered orally to 1,2-dimethylhydrazine (DMH)-induced rats for 16 weeks. The cytotoxicity of both compounds was tested on Caco-2, CT26, and NIH/3T3 cells using the MTT method. The cell cycle, apoptosis, and reactive oxygen species (ROS) levels were analyzed through flow cytometry. X-gal staining was used to examine the compound's effect on senescence. Oral co-administration of CCA-1.1 or PGV-1 significantly suppressed the carcinogenic characteristics and symptoms of premalignant colon cancer relative to DMH-only and untreated groups. CCA-1.1 and PGV-1 administration did not affect the blood profile. CCA-1.1 and PGV-1 demonstrated great cytotoxicity on Caco-2 and CT26 cells, with 50 inhibition concentration (IC 50) values of 4.3 ± 0.2 and 3.1 ± 0.1 μM for CCA-1.1 and 11.2 ± 1.1 and 4.8 ± 0.1 μM for PGV-1, respectively, while not toxic against fibroblast cells. Both compounds instigated G2/M arrest and efficiently induced cell senescence and apoptosis. Moreover, these analogs selectively elevated oxidative stress in colon cancer cells without inducing noticeable changes in fibroblasts. In conclusion, PGV-1 and CCA-1.1 suppressed colorectal tumor formation and induced mitotic arrest. © 2023 Wolters Kluwer Medknow Publications. All rights reserved.

Item Type: Article
Additional Information: Cited by: 4; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: 1,2 dimethylhydrazine; antineoplastic agent; chemoprevention curcumin 1; curcumin; pentagamavunone 1; reactive oxygen metabolite; unclassified drug; animal experiment; animal model; animal tissue; antineoplastic activity; apoptosis; Article; Caco-2 cell line; cell aging; cell cycle; chemoprophylaxis; colorectal cancer; controlled study; CT26 cell line; drug efficacy; erythrocyte count; fibroblast; flow cytometry; G2 phase cell cycle checkpoint; histopathology; in vitro study; in vivo study; inhibitory concentration; leukocyte count; MTT assay; NIH 3T3 cell line; nonhuman; oxidative stress; rat
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 31 Oct 2024 01:37
Last Modified: 31 Oct 2024 01:37
URI: https://ir.lib.ugm.ac.id/id/eprint/6032

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