Wardani, Dian Wahyu and Ningrum, Andriati and Manikharda, Manikharda and Vanidia, Nurul and Munawaroh, Heli Siti Helimatul and Susanto, Eko and Show, Pau-Loke (2023) In silico and in vitro assessment of yellowfin tuna skin (Thunnus albacares) hydrolysate antioxidation effect. Food Hydrocolloids for Health, 3. ISSN 26670259
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Abstract
The protein hydrolysate that contains bioactive peptides of yellowfin tuna (Thunnus albacares) skin collagen with antioxidant activity has been successfully studied by using in silico and in vitro assays. We found that using the in silico assessment, the antioxidant peptides can be found from the precursor (type I α1 and α2 collagen of yellowfin tuna). Applying papain as a protease will also provide the greatest degree of hydrolysis for antioxidative peptides. The highest peptide rank peptides sequence such as Pro-Trp-Gly (PWG), Pro-His-Gly (PHG), His-Leu (HL), Ile-Arg (IR), Ala-His (AH), Glu-Leu (EL) that predicted using papain in silico. Molecular docking analysis showed all peptides derived from yellowfin tuna have hindered the substrate to access the active site of myeloperoxidase (MPO). Interestingly, the substitution of the amino acid from His (PHG, 7.1 kcal/mol) to Trp (PWG, 8.0 kcal/mol) has increased the affinity of the peptide towards MPO. They have antioxidative activities used in silico approach to MPO enzyme. We also confirm the in vitro assays for the protein hydrolysate after proteolysis using papain. The concentration and hydrolysis time will give influence the degree of hydrolysis, and antioxidant activities (P < 0.05). In conclusion, hydrolysate protein of type I α1 and α2 collagen from yellowfin tuna produced by papain hydrolysis has the potential to be used in food, active packaging material until health applications. © 2023 The Authors
Item Type: | Article |
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Additional Information: | Cited by: 9; All Open Access, Gold Open Access |
Uncontrolled Keywords: | antioxidant; collagen; collagen type I alpha 1 chain; collagen type I alpha 2 chain; myeloperoxidase; papain; unclassified drug; amino acid sequence; antioxidant activity; Article; computer model; controlled study; enzyme active site; enzyme substrate; fishery; hydrolysis; in vitro study; molecular docking; nonhuman; protein degradation; skin; Thunnus albacares; tuna |
Subjects: | S Agriculture > S Agriculture (General) |
Divisions: | Faculty of Agricultural Technology > Agro-Industrial Technology |
Depositing User: | Sri JUNANDI |
Date Deposited: | 30 Oct 2024 21:08 |
Last Modified: | 30 Oct 2024 21:08 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/6051 |