Rapid detection of tuna fish oil adulteration using FTIR-ATR spectroscopy and chemometrics for halal authentication

Irnawati, Irnawati and Windarsih, Anjar and Indrianingsih, Anastasia Wheni and Apriyana, Wuri and Ratnawati, Yuli Ary and Nadia, La Ode Muhamad Hazairin and Rohman, Abdul (2023) Rapid detection of tuna fish oil adulteration using FTIR-ATR spectroscopy and chemometrics for halal authentication. Journal of Applied Pharmaceutical Science, 13 (4). 231 – 239. ISSN 22313354

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Abstract

High-quality Tuna fish oil (TFO) is vulnerable to adulteration with lower-quality oil such as pork oil (PO) by unethical players for economic reasons. PO is a nonhalal oil that is prohibited to be consumed by certain religions such as Muslim and Jewish. This research aimed to develop rapid authentication methods using Fourier transform infrared (FTIR) spectroscopy combined with chemometrics for the detection of PO in TFO. FTIR spectroscopy provided fingerprint spectra for TFO and PO. TFO demonstrated two peaks at the carbonyl vibration. The peak at 1,710 cm−1 was specific to TFO whereas the peak at 1,032 cm−1 was specific to PO. Partial least square–discriminant analysis (PLS-DA) and orthogonal projections to latent structures–discriminant analysis (OPLS-DA) were successfully used to detect PO adulteration in TFO by discriminating and classifying TFO and TFO adulterated PO, and PO samples with OPLS-DA showed high accuracy (R2X = 0.998, R2Y = 0.735) and good predictivity (Q2 = 0.552). Meanwhile, the concentration of PO in the mixtures of TFO-PO was successfully predicted using PLS and OPLS with high accuracy (R2 = 0.9928) and good precision. OPLS indicated a better model than PLS with lower error evaluated using root mean square error of estimation (RMSEE) and root mean square error of cross-validation (RMSECV). The obtained RMSEE and RMSECV were 2.81 and 2.94, respectively. This developed method could detect the lowest PO (5) presented in TFO. Therefore, it can be concluded that there was an analytical technique based on FTIR spectra combined with principal component analysis, PLS-DA, and OPLS-DA could be used for halal authentication of TFO. Further, it can be developed for halal authentication of fish oils © 2023 Irnawati Irnawati et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)

Item Type: Article
Additional Information: Cited by: 3; All Open Access, Bronze Open Access
Uncontrolled Keywords: fish oil; Article; attenuated total reflectance Fourier transform infrared spectroscopy; chemometric analysis; controlled study; fish; halal authentication; legal aspect; nonhuman; principal component analysis; receiver operating characteristic; tuna; tuna fish oil adulteration
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 31 Oct 2024 03:30
Last Modified: 31 Oct 2024 03:30
URI: https://ir.lib.ugm.ac.id/id/eprint/6062

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