Antibacterial and cytotoxic secondary metabolites from endophytic fungi associated with Antidesma bunius leaves

Ariantari, Ni Putu and Putra, I Putu Yogi Astara and Leliqia, Ni Putu Eka and Yustiantara, Putu Sanna and Proborini, Meitini Wahyuni and Nugraheni, Nadzifa and Zulfin, Ummi Maryam and Jenie, Riris Istighfari and Meiyanto, Edy (2023) Antibacterial and cytotoxic secondary metabolites from endophytic fungi associated with Antidesma bunius leaves. Journal of Applied Pharmaceutical Science, 13 (7). 132 – 143. ISSN 22313354

Full text not available from this repository. (Request a copy)

Abstract

Endophytic fungi isolated from Antidesma bunius leaves were investigated in this study. Six fungal endophytes were identified as Penicillium steckii AAB-01, Nemania bipapillata AAB-02, Xylaria feejeensis AAB-03, Hypomontagnella monticulosa AAB-04, Daldinia eschscholtzii AAB-05, and Phyllosticta capitalensis AAB-06. All of the isolated endophytic fungi were subjected to fermentation on rice media, followed by extraction with ethyl acetate. When tested for antibacterial activity, P. steckii AAB-01 extract showed the most potent inhibition against Staphylococcus aureus ATCC 6538 and Staphylococcus epidermidis ATCC 12228. Toxicity screening employing brine shrimp lethality test (BSLT) revealed potential toxicity of P. steckii AAB-01 and X. feejeensis AAB-03 extracts. Further investigation showed P. steckii AAB-01 extract had the highest inhibition toward MCF-7 cells, while D. eschscholtzii AAB-05 extract revealed the strongest cytotoxicity against 4T1 cells. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis tentatively identified five compounds, where indole acetic acid, O-acetylharmol, and oxindole I were suggested as the major metabolites in P. steckii AAB-01 extract, while 6-hydroxymelatonin and 2-methyl-6-pentadecylpyridine were suggested as the major metabolites in D. eschscholtzii AAB-05 extract. This is the first report on LC-MS/MS identification of the main metabolites from bioactive extracts of P. steckii AAB-01 and D. eschscholtzii AAB-05 isolated from the leaves of A. bunius, which might contribute to the antibacterial and cytotoxic properties of the extracts. Thus, a detailed investigation of antibacterial and cytotoxic metabolites from these endophytes merits further studies. © 2023 Ni Putu Ariantari et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).

Item Type: Article
Additional Information: Cited by: 3; All Open Access, Gold Open Access
Uncontrolled Keywords: 6 hydroxymelatonin; acetic acid; acetic acid ethyl ester; chloramphenicol; indoleacetic acid; internal transcribed spacer; ketoconazole; plant extract; 4T1 cell line; amino acid sequence; antibacterial activity; Antidesma bunius; antifungal activity; antimicrobial activity; Article; Ascomycetes; Candida albicans; cell viability; cytotoxicity; cytotoxicity test; Daldinia eschscholtzii AAB05; Dothideomycetes; electrospray; endophytic fungus; fermentation; fungus growth; fungus isolation; Fusarium; gene amplification; growth inhibition; human; human cell; Hypomontagnella monticulosa AAB 04; IC50; LC50; lethality; liquid chromatography-mass spectrometry; liquid liquid extraction; mass fragmentography; MCF-7 cell line; medicinal plant; metabolite; minimum inhibitory concentration; mycelium; Nemania bipapillata AAB 02; nonhuman; Penicillium; Penicillium steckii AAB 01; Phyllanthaceae; Phyllosticta capitalensis AAB 06; phylogenetic tree; phylogeny; plant leaf; polymerase chain reaction; Propionibacterium acnes; rice; sequence alignment; shrimp; Staphylococcus aureus; Staphylococcus epidermidis; triple negative breast cancer; Xylaria feejeensis AAB 03
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 01 Nov 2024 02:55
Last Modified: 01 Nov 2024 02:55
URI: https://ir.lib.ugm.ac.id/id/eprint/6072

Actions (login required)

View Item
View Item