Management Diets with Functional Analogue Rice control TCF7L2 Expression of Rattus norvegicus 1769, Berkenhout Model Type 2 Diabetes Mellitus

Type 2 DM is strongly related to TCF7L2 gene that plays a role in the insulin secretion which can be controlled by consuming low glycemic index foods such as functional analogue rice Formula 31 (F31). This study was to determine the effect of functional analogue rice F31 based on mocaf corn, lebui beans and seaweed on TCF7L2 gene expression in rats model of type 2 DM. Male and female rats were induced by streptozotocin at the age of 2 d after birth, then grouped into 4 treatments namely normal BR1 feed (control group), normal functional analogue rice F31, type 2 DM BR1 feed and type 2 DM functional analogue rice F31. TCF7L2 expression was observed through RNA isolation from pancreatic organ, cDNA synthesis and RT-qPCR analysis. The expression of TCF7L2 gene in normal male functional analogue rice F31 and type 2 DM male functional analogue rice F31 was 2.64 times and 1.35 times higher than control respectively. The expression of TCF7L2 in normal female functional analogue rice F31 was 22.01 times higher than control. Management diet with functional analogue rice F31 generally can increase the expression of TCF7L2 gene compared to BR1 feed in rats model of type 2 DM.