Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (<i>Piper Nigrum</i> L) Using A Validated HPLC

The majority of active pharmaceutical ingredients in pharmaceuticals are obtained from natural ingredients, one of which is white pepper. White pepper seeds (Piper nigrum L.) contain an alkaloid compound named piperine, which has a broad spectrum of pharmacological effects. This research aimed to isolate the piperine from white pepper seeds to obtain piperine in a more economical, simple, and effective way so that it can be applied to large-scale production in the pharmaceutical industry. Extraction and purification of piperine were carried out using n-hexane and cyclohexane, respectively. The isolated piperine samples were tested for their purity with a set of melting point apparatus, bidimensional TLC, and HPLC. The compound structure was then analyzed using FTIR and NMR spectroscopy. Cyclohexane was successfully used to remove the resinous matter from the n-hexane extract of white pepper to produce piperine with 94.57 +/- 0.82% of purity. The results of the melting point measurement, FTIR, H-1-NMR, and C-13-NMR spectra of the isolated piperine were similar to that of the piperine reference substance and agreed with data found in the literature.