Pump-Free Microfluidic Device for the Electrochemical Detection of α<sub>1</sub>-Acid Glycoprotein

Sierra, Tania and Jang, Ilhoon and Noviana, Eka and Crevillen, Agustin G. and Escarpa, Alberto and Henry, Charles S. (2021) Pump-Free Microfluidic Device for the Electrochemical Detection of α<sub>1</sub>-Acid Glycoprotein. ACS SENSORS, 6 (8). pp. 2998-3005. ISSN 2379-3694

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Abstract

alpha(1)-Acid glycoprotein (AGP) is a glycoprotein present in serum, which is associated with the modulation of the immune system in response to stress or injuries, and a biomarker for inflammatory diseases and cancers. Here, we propose a pump-free microfluidic device for the electrochemical determination of AGP. The microfluidic device utilizes capillary-driven flow and a passive mixing system to label the AGP with the Os (VI) complex (an electrochemical tag) inside the main channel, before delivering the products to the electrode surface. Furthermore, thanks to the resulting geometry, all the analytical steps can be carried out inside the device: labeling, washing, and detection by adsorptive transfer stripping square wave voltammetry. The microfluidic device exhibited a linear range from 500 to 2000 mg L-1 (R-2 = 0.990) and adequate limit of detection (LOD = 231 mg L-1). Commercial serum samples were analyzed to demonstrate the success of the method, yielding recoveries around 83%. Due to its simplicity, low sample consumption, low cost, short analysis time, disposability, and portability, the proposed method can serve as a point-of-care/need testing device for AGP.

Item Type: Article
Uncontrolled Keywords: capillary-driven microfluidic; passive mixer; alpha(1)-acid glycoprotein; electrochemical detection; electrochemical tag
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 14 Oct 2024 06:51
Last Modified: 14 Oct 2024 06:51
URI: https://ir.lib.ugm.ac.id/id/eprint/9354

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