Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study

Agustika, Dyah K. and Mercuriani, Ixora S. and Ariyanti, Nur Aeni and Purnomo, Chandra W. and Triyana, Kuwat and Iliescu, Daciana D. and Leeson, Mark S. (2021) Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study. SEPARATIONS, 8 (9).

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Abstract

Pepper yellow leaf curl virus (PYLCV) is a threat to chili plants and can significantly reduce yields. This study aimed as a pilot project to detect PYLCV by analyzing compounds emitted by chili plants using gas chromatography-mass spectrometry (GC-MS). The samples investigated in this research were PYLCV-infected and PYLCV-undetected chili plants taken from commercial chili fields. The infection status was validated by using a polymerase chain reaction (PCR) test. A headspace technique was used to extract the volatile organic compounds emitted by plants. The analysis of GC-MS results began with pre-processing, analyzing sample compound variability with a boxplot analysis, and sample classification by using a multivariate technique. Unsupervised multivariate technique principal component analysis (PCA) was performed to discover whether GC-MS could identify PYLCV-infected or not. The results showed that PYLCV-infected and PYLCV-undetected chili plants could be differentiated, with a total percent variance of the first three principal components reaching 91.32%, and successfully discriminated between PYLCV-infected and PYLCV-undetected chili plants. However, more comprehensive studies are needed to find the potential biomarkers of the infected plants.

Item Type: Article
Uncontrolled Keywords: pepper yellow leaf curl virus; plant disease detection; gas chromatography-mass spectrometry; multivariate analysis technique
Subjects: T Technology > TP Chemical technology
Divisions: Faculty of Engineering > Chemistry Engineering Department
Depositing User: Sri JUNANDI
Date Deposited: 14 Oct 2024 06:33
Last Modified: 14 Oct 2024 06:33
URI: https://ir.lib.ugm.ac.id/id/eprint/9370

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