Effectiveness of Moringa oleifera Nanoparticles (Self Nano Emulsifying Drug Delivery System) on Insulin Resistance in the Prediabetes Rattus norvegicus Model

The present study aimed to investigate the effect of Moringa oleifera nanoparticles (MoNP) supplementation based on the Self Nano Emulsifying Drug Delivery System on insulin resistance in prediabetes models. Twenty-five Rattus norvegicus were split into five groups, containing five rats each: normal control group (given a standard diet), as well as a prediabetes control group and three intervention groups fed a high-fat diet for four weeks. Those with fasting blood serum (FBS) ranged betwen 100-130 mg/dL were deemed prediabetic. The intervention groups were then given MoNP at dosages of 75, 125, and 225 mg/kg bw, respectively, whereas the normal and prediabetic groups received a regular diet. FBS, fasting insulin, TNF-α, IL-6, triglycerides, and HOMA-IR were all examined after treatment. MoNP significantly reduced TNF-α, IL-6, triglycerides, and HOMA-IR levels (p<0.05). It also generated a considerable rise in insulin levels in contrast to the prediabetic control group (p<0.05). Supplementation of MoNP at the lowest dose of 75 mg/kg body weight was able to lower FBS to normal levels from 130.04 mg/dL to 97.34 mg/dL and reduce HOMA-IR from 4.20 to 3.71 (p < 0.05). The administration of MoNP can reduce inflammatory cytokines and insulin resistance in the prediabetes model. © 2023 Rusminingsih et al.