Synergistic Antibacterial Effect of Ethyl Acetate Fraction of Vernonia amygdalina Delile Leaves with Tetracycline against Clinical Isolate Methicillin-Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa

Satria, Denny and Harahap, Urip and Dalimunthe, Aminah and Septama, Abdi Wira and Hertiani, Triani and Nasri, Nasri (2023) Synergistic Antibacterial Effect of Ethyl Acetate Fraction of Vernonia amygdalina Delile Leaves with Tetracycline against Clinical Isolate Methicillin-Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. Advances in Pharmacological and Pharmaceutical Sciences, 2023. ISSN 26334682

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Abstract

Multidrug-resistant bacteria have raised global concern about the inability to fight deadly infectious diseases. Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa are the most common resistant bacteria that are causing hospital infections. The present study was undertaken to investigate the synergistic antibacterial effect of the ethyl acetate fraction of Vernonia amygdalina Delile leaves (EAFVA) with tetracycline against the clinical isolates MRSA and P. aeruginosa. Microdilution was used to establish the minimum inhibitory concentration (MIC). A checkerboard assay was conducted for the interaction effect. Bacteriolysis, staphyloxanthin, and a swarming motility assay were also investigated. EAFVA exhibited antibacterial activity against MRSA and P. aeruginosa with a MIC value of 125 μg/mL. Tetracycline showed antibacterial activity against MRSA and P. aeruginosa with MIC values of 15.62 and 31.25 μg/mL, respectively. The interaction between EAFVA and tetracycline showed a synergistic effect against MRSA and P. aeruginosa with a Fractional Inhibitory Concentration Index (FICI) of 0.375 and 0.31, respectively. The combination of EAFVA and tetracycline induced the alteration of MRSA and P. aeruginosa, leading to cell death. Moreover, EAFVA also inhibited the quorum sensing system in MRSA and P. aeruginosa. The results revealed that EAFVA enhanced the antibacterial activity of tetracycline against MRSA and P. aeruginosa. This extract also regulated the quorum sensing system in the tested bacteria. © 2023 Denny Satria et al.

Item Type: Article
Additional Information: Cited by: 3; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: acetic acid ethyl ester; antibiotic agent; Gymnanthemum amygdalinum extract; plant extract; tetracycline; unclassified drug; antibacterial activity; Article; bacteriolysis; cell death; controlled study; drug potentiation; fractional inhibitory concentration index; Gymnanthemum amygdalinum; methicillin resistant Staphylococcus aureus; minimum inhibitory concentration; nonhuman; plant leaf; Pseudomonas aeruginosa; quorum sensing
Subjects: R Medicine > RB Biomedical Sciences
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy
Depositing User: Sri JUNANDI
Date Deposited: 31 Oct 2024 07:45
Last Modified: 31 Oct 2024 07:45
URI: https://ir.lib.ugm.ac.id/id/eprint/6087

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