Lestari, D. and Syamsul, E.S. and Wirnawati, W and Syofyan, S. and Rohman, A. and Hamidi, D. (2023) AUTHENTICATION OF RATTUS NORVEGICUS FAT AND OTHER ANIMAL FATS USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) AND PRINCIPAL COMPONENT ANALYSIS (PCA). International Journal of Applied Pharmaceutics, 15 (Specia). pp. 39-44. ISSN 09757058
AUTHENTICATION-OF-RATTUS-NORVEGICUS-FAT-AND-OTHER-ANIMAL-FATS-USING-GAS-CHROMATOGRAPHYMASS-SPECTROMETRY-GCMS-AND-PRINCIPAL-COMPONENT-ANALYSIS-PCAInternational-Journal-of-Applied-Pharmaceutics.pdf - Published Version
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Abstract
Objective: The objective of this study was to analyze fatty acids using Gas Chromatography-Mass Spectrometry (GC-MS) in combination with chemometric Principal Component Analysis (PCA) for the authentication of Rattus norvegicus fat from other animal fats. Methods: Extraction of fat from raw meat of Rattus norvegicus, beef, chicken, pork, and dogs using the Bligh Dyer method, then derivatized with 0.2 N NaOCH3, precipitation of sodium glycerol was carried out by adding saturated NaCl to obtain methyl esters which were then injected into the GC-MS instrument. The GC-MS data were then processed using chemometric Principal Component Analysis (PCA) to group Rattus norvegicus fat with other animal fats (beef, chicken, pork, and dog). Results: The results of the study revealed that fatty acids in Rattus norvegicus using GC-MS produced eleven types of fatty acids, namely: Lauric acid (1,1), Myristic acid (1,15), Palmitic acid (21,12), Palmitoleic acid (2,06), Stearic acid (8,23), Vaccenic acid (2,43), Oleic acid (26,51), Linoleic acid (19,19), Arachidic acid (0,09), and Eucosatrienoic acid (0,39). Chemometrics Principal Component Analysis (PCA) of Rattus norvegicus fat allows it to be classified with other animal fats. Conclusion: The Gas Chromatography-Mass Spectrometry (GC-MS) method, in combination with chemometric Principal Component Analysis (PCA), offered effective tools for the authentication of fatty acid of Rattus norvegicus. © 2023 The Authors.
Item Type: | Article |
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Additional Information: | cited By 1 |
Uncontrolled Keywords: | arachidic acid; fat; fatty acid; glycerol; lauric acid; linoleic acid; metirosine methyl ester; myristic acid; oleic acid; palmitic acid; palmitoleic acid; sodium; sodium chloride; stearic acid; vaccenic acid, acid hydrolysis; animal cell; animal experiment; animal model; animal tissue; Article; beef; cell line authentication; chicken breed; clinical article; derivatization; dog; mass fragmentography; nonhuman; pork; precipitation; principal component analysis; rat; Rattus norvegicus |
Subjects: | R Medicine > RS Pharmacy and materia medica T Technology > TP Chemical technology |
Divisions: | Faculty of Pharmacy |
Depositing User: | Pujoko PUJOKO |
Date Deposited: | 13 Sep 2024 09:16 |
Last Modified: | 13 Sep 2024 09:16 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/7022 |